The present study explored how gender and a continuous secondary mental task affect reaction time, an essential component of skilled athletic performance. Reaction time is critical for quick, precise decision-making in sports, and reducing it can enhance athletes' success. The study involved 32 healthy, non-athlete students (16 females and 16 males) with an average age of 21.31 ± 1.24 years, who participated in two phases. The first phase consisted of 180 trials with simple, choice, and differential reaction times. In the second phase, a continuous secondary cognitive task with mathematical calculations was added. Results of a mixed ANOVA showed that while the secondary task significantly increased reaction time for both genders (F (1,30) = 92.52, p = 0.001, η2 = 0.496), gender alone did not have a significant effect (p > 0.05), nor was there a significant interaction between gender and the secondary task (p > 0.05). The findings suggest that performing dual tasks increases reaction time due to the higher demand on attention and working memory, though this effect does not differ between males and females. Coaches might consider training methods that mimic cognitive-motor interference to improve athletes' dual-task performance under competition conditions.