The COVID-19 global crisis has left profound psychological impacts on various social groups, in addition to its physical consequences, many of which have persisted into the post-COVID era. Among the consequences of this crisis are its psychological effects on individuals' mental health, which require careful examination. Therefore, the present study aimed to compare psychological readiness, perceived stress, and affect in the psychological well-being of female students in the post-COVID era. The statistical population of this study included 200 female students from Yazd University, aged 19-22 years, who were selected through convenience sampling and completed three questionnaires: the Positive and Negative Affect Schedule , Psychological Readiness, and Perceived Stress Scale. Data analysis was performed using multivariate analysis of variance (MANOVA) in SPSS version 24, with a significance level of 0.05. The results indicated a significant difference between the two groups of students—those who had contracted COVID-19 in the four months prior to the study and those who had not—in the variables of perceived stress and psychological readiness. However, no significant difference was observed in affect and its subscales between the two groups. These findings suggest that the impact of the COVID-19 pandemic on students' stress and psychological readiness persists, while their basic emotions have remained somewhat resilient and stable in the face of this psychological crisis. Thus, these results highlight the importance of addressing stress and psychological readiness in assessing the mental health of students.